Tendon proper- and peritenon-derived progenitor cells have unique tenogenic properties.

IntroductionMultipotent progenitor populations exist within the tendon proper and peritenon of the Achilles tendon. Progenitor populations derived from the tendon proper and peritenon are enriched with distinct cell types that are distinguished by expression of markers of tendon and vascular or pericyte origins, respectively. The objective of this study was to discern the unique tenogenic properties of tendon proper- and peritenon-derived progenitors within an in vitro model. We hypothesized that progenitors from each region contribute differently to tendon formation; thus, when incorporated into a regenerative model, progenitors from each region will respond uniquely. Moreover, we hypothesized that cell populations like progenitors were capable of stimulating tenogenic differentiation, so we generated conditioned media from these cell types to analyze their stimulatory potentials.MethodsIsolated progenitors were seeded within fibrinogen/thrombin gel-based constructs with or without supplementation with recombinant growth/differentiation factor-5 (GDF5). Early and late in culture, gene expression of differentiation markers and matrix assembly genes was analyzed. Tendon construct ultrastructure was also compared after 45 days. Moreover, conditioned media from tendon proper-derived progenitors, peritenon-derived progenitors, or tenocytes was applied to each of the three cell types to determine paracrine stimulatory effects of the factors secreted from each of the respective cell types.ResultsThe cell orientation, extracellular domain and fibril organization of constructs were comparable to embryonic tendon. The tendon proper-derived progenitors produced a more tendon-like construct than the peritenon-derived progenitors. Seeded tendon proper-derived progenitors expressed greater levels of tenogenic markers and matrix assembly genes, relative to peritenon-derived progenitors. However, GDF5 supplementation improved expression of matrix assembly genes in peritenon progenitors and structurally led to increased mean fibril diameters. It also was found that peritenon-derived progenitors secrete factor(s) stimulatory to tenocytes and tendon proper progenitors.ConclusionsData demonstrate that, relative to peritenon-derived progenitors, tendon proper progenitors have greater potential for forming functional tendon-like tissue. Furthermore, factors secreted by peritenon-derived progenitors suggest a trophic role for this cell type as well. Thus, these findings highlight the synergistic potential of including these progenitor populations in restorative tendon engineering strategies

Poincare duality for K-theory of equivariant complex projective spaces

We make explicit Poincare duality for the equivariant K-theory of equivariant complex projective spaces. The case of the trivial group provides a new approach to the K-theory orientation

Comprehensive experimental analysis of nonlinear dynamics in an optically-injected semiconductor laser

We present the first comprehensive experimental study, to our knowledge, of the routes between nonlinear dynamics induced in a semiconductor laser under external optical injection based on an analysis of time-averaged measurements of the optical and RF spectra and phasors of real-time series of the laser output. The different means of analysis are compared for several types of routes and the benefits of each are discussed in terms of the identification and mapping of the nonlinear dynamics. Finally, the results are presented in a novel audio/video format that describes the evolution of the dynamics with the injection parameters. © 2011 Author(s)

Rural Community Participation, Social Networks, and Broadband Use: Examples from Localized and National Survey Data

Although attention has been given to how broadband access is related to economic development in rural areas, scant consideration has been given to how it may be associated with voluntary participation. This issue is important in that numerous studies have shown how much more vital community participation is in rural areas as compared to suburban and urban places. Drawing on three diverse data sets, we examine the influence of broadband access on community participation. In addition, we explore whether broadband access exerts its influence through, in conjunction with, or independent of social networks. The results suggest that broadband access and social network size have independent effects on volunteering in rural places.rural sociology, social networks, broadband, digital inequality, volunteerism, Community/Rural/Urban Development, Research and Development/Tech Change/Emerging Technologies,

Greater Wheeling Regional Plan: Survey Results

The Wheeling Area Strategic Plan included a process for public involvement. Originally, it was to have involved holding open forums and in-person stakeholder meetings. The sessions had been scheduled for early Spring, but they were delayed and eventually cancelled because of the COVID-19 pandemic. Instead, we conducted a web-based survey to gather ideas and opinions from people from across the region

A software architecture for a commander's display system

One of the main tasks of a command and control system is to present commanders with a basis for understanding the tactical situation. A system that transforms a large volume of data into a comprehensive picture that can then be used for situations assessment can aid the commander's decision making process. A system capable of preforming this task has, until recently, required the dedication of vast amounts of computer resources. This report suggests a structure for a commander's display system that relies on recent advances in computional power in the form of a small high-powered, dedicated graphics workstation. A prototype system is included as part of the research effort.Prepared for: Naval Ocean Systems Center, San Diego, CA.http://archive.org/details/softwarearchitec00zydaApproved for public release; distribution is unlimited

Anisotropic dehydration of hydrogel surfaces

Efforts to develop tissue-engineered skin for regenerative medicine have explored natural, synthetic, and hybrid hydrogels. The creation of a bilayer material, with the stratification exhibited by native skin is a complex problem. The mechanically robust, waterproof epidermis presents the stratum corneum at the tissue/air interface, which confers many of these protective properties. In this work we explore the effect of high temperatures on alginate hydrogels, which are widely employed for tissue engineering due to their excellent mechanical properties and cellular compatibility. In particular, we investigate the rapid dehydration of the hydrogel surface which occurs following local exposure to heated surfaces with temperatures in the range 100-200 oC. We report the creation of a mechanically strengthened hydrogel surface, with improved puncture resistance and increased coefficient of friction, compared to the unheated surface. The use of a mechanical restraint during heating promoted differences in the rate of mass loss; the rate of temperature increase within the hydrogel, in the presence and absence of restraint, is simulated and discussed. It is hoped that the results will be of use in the development of processes suitable for preparing skin-like analogues; application areas could include wound healing and skin restoration

Bearing Capacity of Footings on Compacted Sand

This paper presents the results of footing load tests conducted on compacted sand beds to evaluate the bearing capacity and load-displacement characteristics of shallow foundations. Tests were conducted on square concrete footings with widths of 0.30, 0.61, 0.91, and 1.22 m and with embedment ratios (D/B) of 0, 0.5, and 1.0 to investigate the influence of footing size and embedment on the load-displacement behavior and ultimate bearing capacity. A description of the soil and test procedures used is given and the results of the footing load tests are presented. A discussion of the definition of ultimate bearing capacity and the use of normalized curves to describe the footing behavior is presented. A simple model is presented that may prove useful for the design of shallow foundations on sands